What Is It?

Dissolved ozone concentration measures how much active ozone (O₃) is stabilized within the glycerin matrix, expressed in milligrams per liter (mg/L). This is the primary indicator of product potency and therapeutic potential. Higher concentrations mean more active ozone available for biological interaction.

How We Measure It

We use Colorimetric DPD (N,N-diethyl-p-phenylenediamine) analysis. The ozone reacts with the DPD reagent to produce a color change proportional to the ozone concentration. The color intensity is measured with a calibrated spectrophotometer. Our raw meter readings are in PPM, which are then multiplied by 12,500 to determine the actual concentration in the pure glycerin solution.

Why It Matters

Ozone is the active therapeutic compound in ozonated glycerin. A higher dissolved ozone concentration means more potent antimicrobial, anti-inflammatory, and wound-healing properties. Products with low ozone concentrations may look identical but deliver a fraction of the therapeutic benefit.

Our Acceptance Criteria

SimplyO3 Glyzine must contain not less than (NLT) 1,500 mg/L of dissolved ozone. Our batches consistently achieve 2,000–3,000+ mg/L — significantly exceeding the minimum specification.

Industry Comparison

Most competitors in the ozonated glycerin market do not disclose their ozone concentration at all. Of the 10+ brands researched, only SimplyO3 publishes batch-level concentration data. PurO3 (Promolife) and Ozoned Organics both list their concentration as “Not disclosed.” This lack of transparency makes it impossible for consumers to compare product potency — which is exactly why we publish every batch.