Ozone dose quantifies how much ozone was actually delivered to the glycerin during manufacturing. The scientifically defensible metric is Total Ozone Dose = gas-phase ozone concentration × gas flow rate × treatment time, reported in grams of O₃. This measures actual oxidative input rather than relying on time alone, which is a weak surrogate because it doesn’t account for generator output, gas transfer efficiency, or flow rate. References: Gordon et al. (1995); Ferré-Aracil et al. (2015)
SimplyO3 uses industrial 10 g/hr corona discharge ozone generators (German-engineered, 304 SS housing, quartz tube, 316 SS electrode) fed with 99.9% certified medical-grade oxygen at a calibrated flow rate of 1 L/min through 5L and 20L borosilicate glass reaction vessels. At 1 L/min with pure O₂ feed, the gas-phase ozone concentration reaches approximately 165 mg/L (~165 g/m³), delivering ~9.9 g of O₃ per hour to the reactor. A typical 18-hour batch on a 20L vat receives approximately 178 grams of O₃ — an applied dose of ~8,910 mg/L per liter of glycerin.
While we transition to reporting Total Ozone Dose in grams, our current batch records express ozonation as Equivalent Home Generator Hours: raw manufacturing time × 15, based on the output ratio between our industrial generators (~10 g/hr on pure O₂) and a standard home Stratus 3.0 (~0.6 g/hr). For example, 18 raw hours × 15 = 270 equivalent hours. We are implementing gas-phase UV analyzer logging to compute and report exact Total Ozone Dose (grams) per batch as a more traceable and auditable metric.
Run time alone is a weak surrogate for ozone delivery. Delivered dose (concentration × flow × time) integrates gas-liquid mass transfer, ozone demand, and mixing to capture what was actually transferred into the product. In viscous organic media like glycerin, diffuser efficiency, liquid depth, and temperature all influence how much of the measured gas input is absorbed into the batch. Two manufacturers running for the same number of hours can deliver vastly different ozone doses depending on their equipment, oxygen purity, and reactor design.
SimplyO3 Glyzine must achieve not less than (NLT) 200 equivalent hours of ozonation exposure (current metric). Our batches typically achieve 200–900+ equivalent hours. As we transition to Total Ozone Dose reporting, the equivalent acceptance threshold is approximately NLT 130 g O₃ delivered per batch (based on 200 equiv. hrs × 0.66 g/hr effective delivery rate).
Stated accuracy: ±5% on delivered dose. Here is how we calculate and validate ozone delivery:
Three Measured Inputs: Total Ozone Dose = gas-phase O₃ concentration × flow rate × time. Each input has its own uncertainty: (1) gas-phase ozone is measured by UV absorption at 253.7 nm — Ferré-Aracil, Cardona & Navarro-Laboulais (2015) validated the molar extinction coefficient as “3840 ± 109 M⁻¹cm⁻¹” at 258 nm (doi:10.1080/01919512.2014.998756), yielding ~±3% uncertainty; (2) flow is set by calibrated rotameter at 1 L/min ±2%; (3) time is logged electronically with negligible uncertainty.
Combined Uncertainty: These three independent uncertainties combine in quadrature: √(3² + 2² + 0²) ≈ ±3.6%. We round up to ±5% to account for real-world factors: small fluctuations in oxygen feed purity, ambient temperature effects on corona discharge output, and gas transfer losses.
Generator Calibration: Our industrial generators are calibrated monthly. The ozone output (∼165 mg/L at 1 L/min pure O₂) is consistent within ±5% over the service interval, verified by gas-phase UV reading before each production run.
The 15× Multiplier: The equivalent-hours conversion is based on the ratio of our generator output (~10 g O₃/hr) to a standard home Stratus 3.0 (~0.6 g O₃/hr). The true ratio is ~16.7×; we use 15× as a conservative value to avoid overstating. This conversion is a convenience metric for consumer comparison — the underlying grams-of-O₃ calculation is the scientifically defensible number. Ferré-Aracil et al. (2015)
What ±5% Means For Your Batch: If your batch reports 270 equivalent hours, the actual delivered dose is within ±5% of the calculated value — meaning between approximately 257 and 284 equivalent hours. Since our minimum acceptance is 200 equivalent hours, every batch exceeds the threshold with significant margin even at the lower bound of the uncertainty range.