What Is It?

The glycerin assay measures the purity of the glycerin substrate used as the base for ozonation, expressed as a percentage. Higher purity glycerin provides a better medium for ozone stabilization and a longer product shelf life.

How We Test

Testing follows the USP Monograph — Glycerin methodology. This is the United States Pharmacopeia standard for pharmaceutical-grade glycerin, testing for identity, purity, and absence of impurities.

Why It Matters

The quality of the glycerin base directly affects how well ozone is absorbed and retained. Lower-grade glycerin (below 99%) may contain water, organic impurities, or residual processing chemicals that degrade ozone stability and reduce shelf life. Pharmaceutical-grade glycerin provides maximum ozone retention.

Our Standard

SimplyO3 uses exclusively USP-grade pharmaceutical glycerin at ≥99.7% purity. This exceeds the NLT 99.0% acceptance criteria by a significant margin. Our glycerin is sourced from verified pharmaceutical suppliers with full chain-of-custody documentation.

Shelf Life Impact

The combination of high-purity glycerin and extended ozonation gives Glyzine a 90-day unrefrigerated shelf life, 3-year refrigerated shelf life, and indefinite frozen shelf life — far exceeding competitors. Glyzine is kept refrigerated until shipment, so the 90-day clock starts when you receive it. Products using 95–97% glycerin may see ozone degradation within weeks.